Ribonuclease S-peptide as a carrier in fusion proteins
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Ribonuclease S-peptide as a carrier in fusion proteins.
S-peptide (residues 1-20) and S-protein (residues 21-124) are the enzymatically inactive products of the limited digestion of ribonuclease A by subtilisin. S-peptide binds S-protein with high affinity to form ribonuclease S, which has full enzymatic activity. Recombinant DNA technology was used to produce a fusion protein having three parts: carrier, spacer, and target. The two carriers used we...
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The histidine C-2 proton NMR titration curves of ribonuclease S-peptide (residues 1 to 20) and S-protein (residues 21 to 124) are reported. Although S-protein contains 3 histidine residues, four discrete resonances are observed to titrate. One of these arises from the equivalent histidine residues of unfolded S-protein. The variation in area of the four resonances indicate that there is a rever...
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The circular dichroism spectra of pancreatic ribonuclease A, RNase S, and RNase S-protein have been measured in the wave length interval 198 to 300 mp under various conditions. The circular dichroism spectra of RNase A and S are very similar, possibly identical, over this entire wave length interval at neutral pH. That of RNase S-protein is substantially different. It is shown that the portion ...
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Specific regions of the polypeptide sequence of pancreatic ribonuclease have been altered by chemical modification and by limited proteolytic digestion in attempts to implicate specific covalent portions of the molecule in the structure and stabilization of the active center. Digestion of the native molecule with trypsin at elevated temperatures has been shown to remove portions of the chain wi...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2008
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.5560020307